MersV1, Main, Exploration, bibRecord, 002B70

Conformations of DNA strands containing GAGT, GACA, or GAGC tetranucleotide repeats.

Identifieur interne : 002B70 ( Main/Exploration ); précédent : 002B69; suivant : 002B71

Conformations of DNA strands containing GAGT, GACA, or GAGC tetranucleotide repeats.

Auteurs : Jaroslav Kypr [République tchèque] ; Iva Kejnovská ; Michaela Vorlícková

Source :

Biopolymers [ 0006-3525 ] ; 2007.

RBID : pubmed:17680700

Descripteurs français

KwdFr :
- ADN (), Concentration en ions d'hydrogène, Conformation d'acide nucléique, Dichroïsme circulaire, Isomérie, Oligonucléotides (), Répétitions microsatellites, Séquence nucléotidique.
MESH :
- ADN, Concentration en ions d'hydrogène, Conformation d'acide nucléique, Dichroïsme circulaire, Isomérie, Oligonucléotides, Répétitions microsatellites, Séquence nucléotidique.

English descriptors

KwdEn :
- Base Sequence, Circular Dichroism, DNA (chemistry), Hydrogen-Ion Concentration, Isomerism, Microsatellite Repeats, Nucleic Acid Conformation, Oligonucleotides (chemistry).
MESH :
- chemical , chemistry : DNA, Oligonucleotides.
- Base Sequence, Circular Dichroism, Hydrogen-Ion Concentration, Isomerism, Microsatellite Repeats, Nucleic Acid Conformation.

Abstract

The (GA)(n) microsatellite has been known from previous studies to adopt unusual, ordered, cooperatively melting secondary structures in neutral aqueous solutions containing physiological concentrations of salts, at acid pH values or in aqueous ethanol solutions. To find more about the primary structure specificity of these structures, we performed parallel comparative studies of related tetranucleotide repeats (GAGC)(5), (GAGT)(5), and (GACA)(5). The general conclusion following from these comparative studies is that the primary structure specificity is fairly high, indicating that not only guanines but also adenines play a significant role in the stabilization of these unusual structures. (GAGC)(5) is a hairpin or a duplex depending on DNA concentration. Neither acid pH nor ionic strength or the presence of ethanol changed the secondary structure of (GAGC)(5) in a significant way. (GACA)(5) forms a weakly stable hairpin in neutral aqueous solutions but forms a duplex at acid pH where cytosine is protonated. (GAGT)(5) behaves most similar to (GAGA)(5). Salt induces its hairpin to duplex transition at neutral pH and an isomerization into another, probably parallel stranded, duplex takes place at acid pH. (GAGT)(5) is the only of the three present 20-mers that responds to ethanol like (GAGA)(5).

DOI: 10.1002/bip.20822
PubMed: 17680700

Affiliations:

République tchèque

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Conformations of DNA strands containing GAGT, GACA, or GAGC tetranucleotide repeats.</title>
<author><name sortKey="Kypr, Jaroslav" sort="Kypr, Jaroslav" uniqKey="Kypr J" first="Jaroslav" last="Kypr">Jaroslav Kypr</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biophysics, v. v. i., Academy of Sciences of the Czech Republic, Královopolská 135, CZ-612 65 Brno, Czech Republic.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>Institute of Biophysics, v. v. i., Academy of Sciences of the Czech Republic, Královopolská 135, CZ-612 65 Brno</wicri:regionArea>
<wicri:noRegion>CZ-612 65 Brno</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kejnovska, Iva" sort="Kejnovska, Iva" uniqKey="Kejnovska I" first="Iva" last="Kejnovská">Iva Kejnovská</name>
</author>
<author><name sortKey="Vorlickova, Michaela" sort="Vorlickova, Michaela" uniqKey="Vorlickova M" first="Michaela" last="Vorlícková">Michaela Vorlícková</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2007">2007</date>
<idno type="RBID">pubmed:17680700</idno>
<idno type="pmid">17680700</idno>
<idno type="doi">10.1002/bip.20822</idno>
<idno type="wicri:Area/PubMed/Corpus">002168</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002168</idno>
<idno type="wicri:Area/PubMed/Curation">002168</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002168</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002073</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002073</idno>
<idno type="wicri:Area/Ncbi/Merge">000538</idno>
<idno type="wicri:Area/Ncbi/Curation">000538</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000538</idno>
<idno type="wicri:doubleKey">0006-3525:2007:Kypr J:conformations:of:dna</idno>
<idno type="wicri:Area/Main/Merge">002B96</idno>
<idno type="wicri:Area/Main/Curation">002B70</idno>
<idno type="wicri:Area/Main/Exploration">002B70</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Conformations of DNA strands containing GAGT, GACA, or GAGC tetranucleotide repeats.</title>
<author><name sortKey="Kypr, Jaroslav" sort="Kypr, Jaroslav" uniqKey="Kypr J" first="Jaroslav" last="Kypr">Jaroslav Kypr</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biophysics, v. v. i., Academy of Sciences of the Czech Republic, Královopolská 135, CZ-612 65 Brno, Czech Republic.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>Institute of Biophysics, v. v. i., Academy of Sciences of the Czech Republic, Královopolská 135, CZ-612 65 Brno</wicri:regionArea>
<wicri:noRegion>CZ-612 65 Brno</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kejnovska, Iva" sort="Kejnovska, Iva" uniqKey="Kejnovska I" first="Iva" last="Kejnovská">Iva Kejnovská</name>
</author>
<author><name sortKey="Vorlickova, Michaela" sort="Vorlickova, Michaela" uniqKey="Vorlickova M" first="Michaela" last="Vorlícková">Michaela Vorlícková</name>
</author>
</analytic>
<series><title level="j">Biopolymers</title>
<idno type="ISSN">0006-3525</idno>
<imprint><date when="2007" type="published">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term>
<term>Circular Dichroism</term>
<term>DNA (chemistry)</term>
<term>Hydrogen-Ion Concentration</term>
<term>Isomerism</term>
<term>Microsatellite Repeats</term>
<term>Nucleic Acid Conformation</term>
<term>Oligonucleotides (chemistry)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN ()</term>
<term>Concentration en ions d'hydrogène</term>
<term>Conformation d'acide nucléique</term>
<term>Dichroïsme circulaire</term>
<term>Isomérie</term>
<term>Oligonucléotides ()</term>
<term>Répétitions microsatellites</term>
<term>Séquence nucléotidique</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA</term>
<term>Oligonucleotides</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Circular Dichroism</term>
<term>Hydrogen-Ion Concentration</term>
<term>Isomerism</term>
<term>Microsatellite Repeats</term>
<term>Nucleic Acid Conformation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>ADN</term>
<term>Concentration en ions d'hydrogène</term>
<term>Conformation d'acide nucléique</term>
<term>Dichroïsme circulaire</term>
<term>Isomérie</term>
<term>Oligonucléotides</term>
<term>Répétitions microsatellites</term>
<term>Séquence nucléotidique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The (GA)(n) microsatellite has been known from previous studies to adopt unusual, ordered, cooperatively melting secondary structures in neutral aqueous solutions containing physiological concentrations of salts, at acid pH values or in aqueous ethanol solutions. To find more about the primary structure specificity of these structures, we performed parallel comparative studies of related tetranucleotide repeats (GAGC)(5), (GAGT)(5), and (GACA)(5). The general conclusion following from these comparative studies is that the primary structure specificity is fairly high, indicating that not only guanines but also adenines play a significant role in the stabilization of these unusual structures. (GAGC)(5) is a hairpin or a duplex depending on DNA concentration. Neither acid pH nor ionic strength or the presence of ethanol changed the secondary structure of (GAGC)(5) in a significant way. (GACA)(5) forms a weakly stable hairpin in neutral aqueous solutions but forms a duplex at acid pH where cytosine is protonated. (GAGT)(5) behaves most similar to (GAGA)(5). Salt induces its hairpin to duplex transition at neutral pH and an isomerization into another, probably parallel stranded, duplex takes place at acid pH. (GAGT)(5) is the only of the three present 20-mers that responds to ethanol like (GAGA)(5).</div>
</front>
</TEI>
<affiliations><list><country><li>République tchèque</li>
</country>
</list>
<tree><noCountry><name sortKey="Kejnovska, Iva" sort="Kejnovska, Iva" uniqKey="Kejnovska I" first="Iva" last="Kejnovská">Iva Kejnovská</name>
<name sortKey="Vorlickova, Michaela" sort="Vorlickova, Michaela" uniqKey="Vorlickova M" first="Michaela" last="Vorlícková">Michaela Vorlícková</name>
</noCountry>
<country name="République tchèque"><noRegion><name sortKey="Kypr, Jaroslav" sort="Kypr, Jaroslav" uniqKey="Kypr J" first="Jaroslav" last="Kypr">Jaroslav Kypr</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B70 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002B70 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:17680700
   |texte=   Conformations of DNA strands containing GAGT, GACA, or GAGC tetranucleotide repeats.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:17680700" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

Serveur d'exploration MERS

Conformations of DNA strands containing GAGT, GACA, or GAGC tetranucleotide repeats.

Conformations of DNA strands containing GAGT, GACA, or GAGC tetranucleotide repeats.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.